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Promega E. coli S30 Extract System for Linear Templates

The E. coli S30 Extract System for Linear Templates allows transcription/translation of linear DNA templates.

Supplier:  Promega L1030

Catalog No. PR-L1030

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Includes: S30 Extract, Linear, 3 × 150μL; Luciferase Dilution Reagent, 1mL; pBESTluc™ DNA, Linear (Control), 10μg; Luciferase Assay Reagent, 250μL; S30 Premix without amino acids, 750μL; Amino acid mixtures, 3 × 175μL



  • Successful Transcription/Translation of Linear DNA Templates


Identification of cloned or synthesized genes from linear DNA, rapid structure mapping of genes without additional cloning steps, rapid verification of in-vitro-generated mutations, synthesis of truncated gene products for functional domain mapping and epitope mapping studies, expression from in-vitro-generated RNA for genes lacking an appropriate E. coli promoter
3 x 150μL S30 linear extract, 1mL Luciferase Dilution Reagent, 10μg pBESTluc™ DNA, Linear (control), 250μL Luciferase Assay Reagent, 750μL S30 premix without Amino A


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