Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More

Invitrogen™ pLenti6.3/V5™-TOPO™ TA Cloning™ Kit

pLenti6.3/V5™-TOPO™ TA Cloning™ Kit

Supplier:  Invitrogen™ K531520

Encompass

Catalog No. K531520


Add to cart

Description

Description

Invitrogen's™ pLenti6.3⁄V5™-TOPO™Cloning Kit is part of our ViraPower™ HiPerform™ Lentiviral Expression System (catalog K5310-00).

The pLenti6.3⁄V5™-TOPO™ -TA Cloning Kit contains the TOPO™- adapted ViraPower™ HiPerform™ lentiviral expression vector, pLenti6.3⁄V5™-TOPO™ for quick PCR-based cloning and high-level expression of a target gene in dividing and non-dividing mammalian cells. The pLenti6.3⁄V5™-TOPO™ vector is equipped with two key genetic elements, making it a HiPerform™ vector: the Woodchuck Posttranscriptional Regulatory Element (WPRE) and the central Polypurine Tract (cPPT) sequence from the HIV-1 integrase gene to produce at least 4-fold increase in protein expression compared to vectors lacking these elements.

Advantages
  • Stable expression
  • Long-term experiments
  • Accurate titer of functional virus
  • 5 minute, high-efficiency TOPO™ PCR cloning in three simple steps

    Key Features
  • HiPerform™ WPRE and cPPT elements
  • CMV promoter
  • V5 epitope tag at C terminus
  • Blasticidin selection

    Kit includes
  • A lacZ vector as a positive control, pLenti6.3⁄V5-GW⁄lacZ™
  • Stbl3™ competent cells

    For research use only. Not intended for any therapeutic or diagnostic use.
  • Specifications

    Specifications

    Competent Cell
    Constitutive
    Lentiviral
    ViraPower™ HiPerform™ Lentiviral Expression Systems
    Viral Expression
    Cloning Kit
    V5 Epitope Tag
    SV40 Promoter
    TOPO-TA
    TOPO™ TA Cloning Reagents (Box 1)
    pLenti6.3?V5™-TOPO™ (5-10 ng⁄µL): 20 µL, -20°C
    pLenti6.3⁄V5-GW⁄lacZ™ Control vector (0.5 µg⁄µL): 20 µL, -20°C
    10X PCR Buffer: 100 µL, -20°C
    dNTP Mix: 10 µL, -20°C
    Salt Solution: 50 µL, -20°C
    CMV Forward Primer (100 ng⁄µL): 20 µL, -20°C
    V5 (C-term) Reverse Primer (100 ng⁄µL): 20 µL, -20°C
    Control PCR Template (50 ng⁄µL): 10 µL, -20°C
    Control PCR Primers (100 ng⁄µL): 10 µL, each -20°C
    Sterile Water: 1 mL

    One Shot™ Stbl3™ Chemically Competent E. coli (Box 2)
    Stbl3™ Cells: 21 x 50 µL, -80°C
    pUC19 control DNA (10 pg⁄µL): 50 µL, -80°C
    S.O.C. Medium: 6 mL, -80°C
    Kit
    Protein Expression
    HiPerform™, TOPO™, ViraPower™
    CMV
    Blasticidin
    pLenti, TOPO-TA Vector
    SDS
    Documents

    Documents

    Product Certifications
    Promotions

    Promotions

    Provide Content Correction

    The Fisher Scientific Encompass Program offers items which are not part of our distribution portfolio. These products typically do not have pictures or detailed descriptions. However, we are committed to improving your shopping experience. Please use the form below to provide feedback related to the content on this product.

    Product Title

    By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

    Cancel Submit

    For Research Use Only. Not for use in diagnostic procedures.