Thermo Scientific™ Pierce™ RNA 3' End Desthiobiotinylation Kit

Perform easy and efficient desthiobiotin labeling of RNA for use as probes or as antibody alternatives for enrichment of RNA binding proteins.

Supplier:  Thermo Scientific™ 20163

Catalog No. PI20163

Description

Perform easy and efficient desthiobiotin labeling of RNA for use as probes or as antibody alternatives for enrichment of RNA binding proteins with Thermo Scientific™ Pierce RNA 3' End Desthiobiotinylation Kit.

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The Pierce RNA 3'-End Desthiobiotinylation Kit is optimized for labeling the 3'-end of single-stranded RNA using T4 RNA ligase. Once labeled, the RNA can be used as a probe or target for gel-shift EMSA reactions, Northern blots and protein-RNA interaction experiments. The desthiobiotinylated RNA also can be used to enrich for RNA binding proteins (RBP) using streptavidin affinity resin, because the desthiobiotin tag binds to streptavidin in a manner which allows gentle elution of the ribonucleoprotein complex. The labeling kit is also included as a component of the Thermo Scientific Pierce Magnetic RNA-Protein Pull-Down Kit.

Highlights:

• Non-radioactive

  – a single biotin labels has similar detection sensitivity as radioactivity

• Easy to use – RNA ligase and optimized reaction buffer are included

• Economical – only a fraction of the cost purchasing synthetic biotinylated RNA probes

• End-labeled – results in minimal disturbance of RNA secondary structure

• Flexible

  – desthiobiotin label may be used for detection or as an affinity handle for streptavidin resin

Includes:

• Desthiobiotinylated cytidine bisphosphate, T4 RNA ligase and buffer, unlabeled RNA oligonucleotide for use as a positive control, a biotinylated RNA probe standard, RNase inhibitor, glycogen, and ligation enhancing reagents

Recommended for:

• Northern blotting
• EMSA
• Enrichment of RNA binding proteins (RBP)

Specifications

• Quantity: 20 Reactions
• Isolation Technology: Magnetic Bead
• Product Line: Pierce™
• Format: Kit

• Content And Storage: Sufficient For: 20 desthiobiotinylation reactions using 50 pmol of RNA each
  • Desthiobiotinylated Cytidine Bisphosphate, 40 µL
  • T4 RNA Ligase, 40 µL
  • T4 RNA Ligase Reaction buffer (10X), 100 µL
  • Non-labeled RNA Control, 100 µL
  • Biotinylated IRE RNA Control, 35 µL
  • RNase Inhibitor, 2 x 10 µL
  • DMSO, 200 µL
  • PEG (30%), 300 µL
  • Glycogen, 20 µL
  • Nuclease-free Water, 1.5 mL
  
  Upon receipt, store at -20°C.
• No. of Reactions: 20
• Product Type: RNA Labeling Kit