Thermo Scientific™ Pierce™ RNA 3' End Desthiobiotinylation Kit
Perform easy and efficient desthiobiotin labeling of RNA for use as probes or as antibody alternatives for enrichment of RNA binding proteins.
Supplier: Thermo Scientific™ 20163
Perform easy and efficient desthiobiotin labeling of RNA for use as probes or as antibody alternatives for enrichment of RNA binding proteins with Thermo Scientific™ Pierce RNA 3' End Desthiobiotinylation Kit.
– a single biotin labels has similar detection sensitivity as radioactivity
Easy to use – RNA ligase and optimized reaction buffer are included
Economical – only a fraction of the cost purchasing synthetic biotinylated RNA probes
End-labeled – results in minimal disturbance of RNA secondary structure
– desthiobiotin label may be used for detection or as an affinity handle for streptavidin resin
- Desthiobiotinylated cytidine bisphosphate, T4 RNA ligase and buffer, unlabeled RNA oligonucleotide for use as a positive control, a biotinylated RNA probe standard, RNase inhibitor, glycogen, and ligation enhancing reagents
- Northern blotting
- Enrichment of RNA binding proteins (RBP)
|Sufficient For: 20 desthiobiotinylation reactions using 50 pmol of RNA each
• Desthiobiotinylated Cytidine Bisphosphate, 40 µL
• T4 RNA Ligase, 40 µL
• T4 RNA Ligase Reaction buffer (10X), 100 µL
• Non-labeled RNA Control, 100 µL
• Biotinylated IRE RNA Control, 35 µL
• RNase Inhibitor, 2 x 10 µL
• DMSO, 200 µL
• PEG (30%), 300 µL
• Glycogen, 20 µL
• Nuclease-free Water, 1.5 mL
Upon receipt, store at -20°C.
|RNA Labeling Kit|
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