Thermo Scientific™ Pierce™ Protein A UltraLink™ Resin

Extremely durable polyacrylamide support with immobilized Protein A for standard-capacity IgG purification.

Supplier:  Thermo Scientific™ 53139

Catalog No. PI53139

Description

Thermo Scientific™ Pierce Protein A UltraLink Resin is an extremely durable beaded polyacrylamide support to which IgG-binding Protein A has been immobilized for use in IgG purification methods.

The Protein A UltraLink Resin is used to bind and purify IgG in gravity-flow, spin-column or medium-pressure chromatography methods. Protein A is a popular antibody-binding protein that is especially useful for affinity purification of IgG from rabbit or human serum.

UltraLink Resin is an azlactone-activated polyacrylamide support that is hydrophilic, charge-free, high capacity, highly crosslinked, rigid and porous. This support is especially useful for medium pressure techniques when using large sample volumes requiring fast-flow techniques (FPLC) and large-scale applications. Unlike typical beaded agarose supports, UltraLink Support does not crush or collapse when subjected to high flow rates that result in pressures exceeding 25psi.

Highlights:

• Immobilized Protein A – specifically binds IgG from several mammalian species, especially most subtypes of human and rabbit IgG

• Standard capacity – loading of immobilized Protein A provides a binding capacity of approx. 16mg human IgG per milliliter of resin

• UltraLink Resin – acrylamide-based support provides an alternative to crosslinked beaded agarose supports for medium pressure applications

Properties of Thermo Scientific UltraLink Resin:

• Support pH Stability: 1 to 13
• Average Particle Size: 50 to 80 microns
• Exclusion Limit: greater than 2,000,000 daltons
• Average Surface Area: greater than 250m2 per gram of beads
• Average Pore Volume: greater than 1.2mL per gram of beads (> 60% of bead volume)
• Pore Size: 500 to 1000 angstroms (50 to 100nm)
• Maximum Linear Velocity: 3000cm per hour
• Maximum Pressure: 100psi (6.9 bar), defined as the maximum pressure drop across a column that the resin can withstand (Note: The indicated gauge pressure of a liquid chromatography apparatus may be measuring the total system pressure rather than the pressure drop across the column.)

Specifications

• Product Type: Resin
• Column Type: UltraLink Affinity Resin
• Stationary Phase: Protein A
• Content And Storage: Upon receipt store products at 4°C. Products are shipped at ambient temperature.

• Product Line: Pierce™, UltraLink™
• Format: Bottle
• Purification Target: IgG Antibodies
• Quantity: 5 mL

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.