Thermo Scientific™ Metal Enhanced DAB Substrate Kit

Special formulation of diaminobenzidine peroxidase substrate that uses cobalt and nickel chloride to yield intense color for IHC staining.

Supplier:  Thermo Scientific™ 34065

Catalog No. PI34065

Description

Thermo Scientific™ Pierce Metal-Enhanced DAB Substrate Kit uses cobalt and nickel chloride in a special formulation of diaminobenzidine peroxidase substrate that yields intense color for immunohistochemical staining.

The color produced by DAB (3,3'-diaminobenzidine) can be intensified by the addition of metals such as nickel, copper, silver and cobalt that form more dense complexes upon reaction in HRP-peroxide substrate reactions. The near-black signal (precipitate) is easy to photograph.

Highlights:

• Incredible sensitivity – 50 times more sensitive than the traditional DAB method and 30 times more sensitive than other metal-intensified versions of DAB

• Low background, high intensity – get a crisp dark brown-black precipitate and, even with the increased intensity, background is almost nonexistent

• Six-hour stability – working solution is stable for more than six hours at room temperature

Includes:

Metal Enhanced DAB (10X), 25mL; Stable Peroxide Buffer, 250mL

Specifications

• Detection Method: Chromogenic, Colorimetric
• Content And Storage:
  • Metal Enhanced DAB (10X), 25 mL (store at -20°C)
  • Stable Peroxide Buffer, 250 mL (store at 4°C)
• Substrate Type: HRP (Horseradish Peroxidase) Substrate
• For Use With (Application): Immunodetection, IHC (Immunohistochemistry)

• Quantity: 275 mL of 1X working solution
• Substrate: DAB (Enhanced)
• Product Line: Pierce™
• Product Type: DAB Substrate Kit

For Research Use Only. Not for use in diagnostic procedures.