Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More
Learn More
Description
OPTIZYME™ T4 DNA Pol is a template-dependent DNA polymerase that catalyzes 5'->3' synthesis from primed single-stranded DNA.
Applications and features:
- OPTIZYME T4 DNA Polymerase creates blunt DNA ends by filling in 5'-overhangs and/or removing 3'-overhangs
- Creation of blunt PCR products with 3'-dA overhangs
- Oligonucleotide-directed site-specific mutagenesis
- Synthesis of labeled DNA probes (by replacement reaction)
- Ligation-independent cloning of PCR products
- Active in buffers for OPTIZYME restriction enzymes, reverse transcriptases and T4 DNA ligase
- 3'->5' exonuclease activity
- Lacks 5'->3' exonuclease activity
Supplied with:
5X OPTIZYME T4 DNA Pol Buffer: 335mM Tris-HCl (pH 8.8 at 25°C), 33mM MgCl2, 5mM DTT, 84mM (NH4)2SO4
Source: E. Coli cells with a cloned gene 43 of bacteriophage T4
Quality Control:
Endodeoxyribonuclease-free
Specifications
5 U/μL | |
7.5 | |
OPTIZYME™ T4 DNA Pol Buffer 5X: Tris HCl 335mM (8.8 pH at 25°C), MgCl2 33mM, DTT 5mM, (NH4)2SO4 84mM | |
E. coli cells with a cloned gene 43 of bacteriophage T4 | |
T4 DNA Polymerase |
Colorless | |
Synthesis of labeled DNA probes, creation of blunt PCR products with 3'-dA overhangs, ligation-independent cloning of PCR products, Restriction Enzymes, reverse transcriptases and T4 DNA ligase | |
-20°C | |
100 U | |
Liquid |
Provide Content Correction
We continue to work to improve your shopping experience and your feedback regarding this content is very important to us. Please use the form below to provide feedback related to the content on this product.
Product Title
Spot an opportunity for improvement?
Provide Content Correction