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OPTIZYME™ BamHI, Fisher BioReagents™
Specifications
Concentration | 10 U/μL |
---|---|
Components | 25 to 50% Water, >50% Glycerin, 1 to 2.5% Sodium Chloride |
Incubator Temperature | 37°C |
pH | 7.4 |
For Use With (Application) | >95% of DNA fragments can be ligated and re-cut after 50-fold over-digestion with BamHI |
Description
5'...G^G A T C C...3'
3'...C C T A G^G...5'
Conditions for 100% Activity:
- 1X OPTIZYME Buffer BamHI: 10mM Tris-HCl (pH 8.0 at 37°C), 5mM MgCl2, 100mM KCl, 0.02% Triton X-100 and 0.1mg/ml BSA.
- Incubate at 37°C
Enzyme Activity in OPTIZYME buffers:
- Buffer 1: 20 - 50%™
- Buffer 2: 100%
- Buffer 3: 20 - 50%
- Buffer 4: 100%™
- Buffer 5: 50 - 100%™
Storage Buffer:
10mM Tris-HCl (pH 7.4 at 25°C), 200mM NaCl, 1mM DTT, 0.1mM EDTA, 0.15% Triton X-100, 0.2mg/ml BSA and 50% (v/v) glycerol.
Ligation and Recleavage:
More than 95% of DNA fragments can be ligated and recut after a 50-fold overdigestion with BamHI.
Digestion of Agarose-embedded DNA:
A minimum of 5 units of BamHI is required for the complete digestion of 1μg of agarose-embedded lambda DNA in 16 hours.
Note: Low salt, high glycerol (>5%) concentrations, pH >8.0 or a large excess of enzyme may result in star activity.
Compatible Ends: BclI, BglII, Bsp143I, MboI, PsuI.
Methylation Effects:
Dam: completely overlaps - no effect.
Dcm: may overlap - no effect.
CpG: may overlap - no effect.
EcoKI: never overlaps - no effect.
EcoBI: never overlaps - no effect.
Specifications
10 U/μL | |
37°C | |
>95% of DNA fragments can be ligated and re-cut after 50-fold over-digestion with BamHI | |
Keep container tightly closed | |
BamHI |
25 to 50% Water, >50% Glycerin, 1 to 2.5% Sodium Chloride | |
7.4 | |
10mM Tris HCl (pH 7.4 at 25°C), 200mM NaCl, 1mM DTT, 0.1mM EDTA, 0.15% Triton™ X-100, 0.2mg/mL BSA, 50% Glycerol | |
G.GATCC | |
Liquid |
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