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Invitrogen™ Novex™ 10 to 20%, Tricine, 1.0 mm, Mini Protein Gels

Invitrogen Novex Tricine Gels provide separation of low molecular weight proteins and peptides. In this system tricine replaces glycine in the running buffer, resulting in more efficient stacking and destacking for low molecular weight proteins and higher resolution of smaller peptides.

$253.00

Specifications

For Use With (Equipment) Mini Gel Tank, XCell SureLock Mini-Cell
Gel Percentage 10 to 20%
Gel Size Mini
Gel Thickness 1.0 mm
Gel Type Tricine
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Products 3
Catalog Number Mfr. No. Wells Price Quantity & Availability  
Catalog Number Mfr. No. Wells Price Quantity & Availability  
EC6625BOX
Encompass_Preferred
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Invitrogen™
EC6625BOX
10-well
Case for $253.00
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EC66252BOX
Encompass_Preferred
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Invitrogen™
EC66252BOX
12-well
Case for $253.00
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EC66255BOX
Encompass_Preferred
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Invitrogen™
EC66255BOX
15-well
Case for $253.00
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Description

Description

Invitrogen Novex Tricine Gels provide separation of low molecular weight proteins and peptides. The Tricine system is a modification of the tris-glycine discontinuous buffer system developed by Schaegger and von Jagow (Schaegger and von Jagow, 1987) specifically for resolving peptides and low molecular weight proteins. In this system the tricine replaces the glycine in the running buffer, resulting in more efficient stacking and destacking for low molecular weight proteins and higher resolution of smaller peptides.

Features of Novex Tricine protein gels:
• Increased resolution of proteins with molecular weights as low as 2 kDa
• Improved compatibility with direct sequencing of proteins after transferring to PVDF
• Minimized protein modification due to the lower pH of the tricine buffering system

Formulation
Invitrogen Tricine gels are made with high-purity, strictly quality-controlled reagents: Tris base, HCl, acrylamide, bisacrylamide, TEMED, APS, and highly purified water. Our Tricine gels have a 4% stacking gel and do not contain SDS. The Tricine system requires SDS in sample and running buffers for best results.

Choose the right Tricine gel for your protein separation
Invitrogen Tricine gels come in three polyacrylamide concentrations of 10%, 16%, and a gradient of 10–20%. Select from our many well formats, including 10-, 12-, and 15-well. Tricine gels are formulated for denaturing gel electrophoresis applications. For optimal sample preparation, we recommend Tricine SDS Sample Buffer (LC1676) and optimal separation use Tricine SDS Running Buffer (LC1675).

For transfer of proteins to a membrane, we recommend using the Novex Tris-Glycine Transfer Buffer (LC3675) if performing a traditional wet transfer using the XCell II Blot Module (EI9051) or the Mini Blot Module (B1000). Rapid semi-dry transfer can be performed using the Invitrogen Power Blotter or rapid dry transfer using the iBlot 2 Gel Transfer Device (IB21001).

Specifications

Specifications

Mini Gel Tank, XCell SureLock Mini-Cell
Mini
Tricine
2.5 to 250 kDa
Molecular Weight
10 Gels/Box
Store at 2°C to 8°C. Do not freeze.
8 cm
10 to 20%
1.0 mm
Denaturing
Denaturing
Novex™
Wet Ice
8 cm
Safety and Handling

Safety and Handling

ShelfLife : 16 Weeks

SDS
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