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MilliporeSigma™ Novagen™ T7•Tag™ Affinity Purification Kit
Rapid immunoaffinity purification of target proteins that carry the T7•Tag sequence
Supplier: MilliporeSigma™ 690253
Description
- Designed for rapid immunoaffinity purification of target proteins that carry the T7•Tag sequence
- Purification is based on binding target proteins to T7•Tag Monoclonal Antibody that is covalently coupled to cross-linked agarose beads, washing away unbound proteins, and eluting at pH 2.2
- Neutralization Buffer is included to limit protein exposure to low pH
- Capacity will vary somewhat between different target proteins, but the beads are standardized to bind a minimum of 300μg T7•Tag β-galactosidase per milliliter of settled resin
- The beads can be used in either batch or column methods and can be regenerated a minimum of five times without loss of binding activity
Specifications
Store at +2°C to +8°C | |
1 Kit |
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