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MilliporeSigma™ Novagen™ BLR (DE3) pLysS Competent Cells

BLR is a recA derivative of BL21 that improves plasmid monomer yields and may help stabilize target plasmids containing repetitive sequences. T7 lysozyme expression suppresses basal T7 expression.

$175.00 - $315.00

Specifications

Efficiency Guaranteed efficiency: >2 x 106 cfu/μg
Content And Storage ≤-70°C
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Products 2
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
69-956-3
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MilliporeSigma™
699563
0.4 mL
Each for $175.00
Add to Cart
 
69-956-4
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MilliporeSigma™
699564
1 mL
Each for $315.00
Add to Cart
 
Description

Description

BLR is a recA- derivative of BL21 that improves plasmid monomer yields and may help stabilize target plasmids containing repetitive sequences or whose products may cause the loss of the DE3 prophage (1, 2). DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG. pLysS strains express T7 lysozyme, which further suppresses basal expression of T7 RNA polymerase prior to induction, thus stabilizing pET recombinants encoding target proteins that affect cell growth and viability.
Specifications

Specifications

Guaranteed efficiency: >2 x 106 cfu/μg
≤-70°C
SDS
Documents

Documents

Product Certifications
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