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Thermo Scientific™ North2South™ Chemiluminescent Hybridization and Detection Kit
Acquire consistent, highly sensitive Southern blot results with a novel ECL HRP substrate and optimized hybridization and blocking buffers.
Supplier: Thermo Scientific™ 17097
Description
Thermo Scientific™ North2South Chemiluminescent Detection Kit combines a novel ECL substrate for horseradish peroxidase (HRP) and optimized hybridization and blocking buffers to ensure consistent, high-sensitivity Southern blot results.
The Thermo Scientific North2South Hybridization and Detection Kit combines a novel enhanced luminol substrate for horseradish peroxidase (HRP) with optimized pre-made hybridization and blocking buffers to ensure consistent results and high sensitivity. Any nucleic acid blot can be hybridized with the appropriate biotinylated (biotin-labeled) oligonucleotide probe. After a brief membrane blocking step, the biotin label is detected with streptavidin-HRP and a 5-minute incubation with the chemiluminescent substrate. Results are easily recorded by brief exposure to X-ray film (1-15 minutes) or CCD camera.Highlights:
- Sensitivity equal to or greater than 32P
- Amenable to many formats
- High level of light output and consistent low background allows for very short exposure times using cooled CCD camera systems or film
- Ready-to-use buffers and short process time makes switching from radioactive detection to chemiluminescent detection an easy transition, even for the novice user
Specifications
Sufficient For: 1000 cm2 of membrane (10 mini-gel blots) • Stabilized Streptavidin-HRP, 1.5 mL • Luminol/Enhancer Solution, 80 mL • Stable Peroxide Solution, 80 mL • Hybridization Buffer, 125 mL • Hybridization Stringency Wash Buffer (2X), 375 mL • Blocking Buffer, 500 mL • Wash Buffer (4X), 500 mL • Substrate Equilibration Buffer, 500 mL Upon receipt, store kit at 4°C. |
|
Kit | |
Hybridization and Detection Kit |
Chromogenic | |
North2South™ | |
2160 mL Kit |
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