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Invitrogen™ Di-2-ANEPEQ (JPW 1114)

Molecules that fluoresce in response to electrical potential changes in their environment

Supplier:  Invitrogen™ D6923


Catalog No. D6923

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  • Fast-response probes that operate by means of change in their electronic structure, and consequently their fluorescence properties, in response to change in surrounding electric field
  • Optical response is sufficiently fast to detect transient (millisecond) potential changes in excitable cells, including single neurons, cardiac cells, and intact brains
  • Magnitude of their potential-dependent fluorescence change is often small; fast-response probes typically show 2-10% fluorescence change per 100mV
  • Display potential-dependent shift in their excitation spectra, thus permitting quantitation of membrane potential using excitation ratio measurements
  • Cationic molecule; exhibits very low internalization and good signal:noise ratio and is useful for neural network analysis
  • Ex/Em maxima bound to model phospholipid membranes are approximately 465/635nm (but spectral properties are highly dependent on environment)
  • Nonfluorescent until bound to membranes
  • Cationic molecule, soluble in water
  • Detects submillisecond membrane potential changes
  • Dye is introduced into cells by direct addition of stock solution to cell culture medium, by using by retrograde labeling
  • Plasma membrane of cell has transmembrane potential of approximately -70mV (negative inside) as a consequence of K+, Na+, and Cl- concentration gradients that are maintained by active transport processes
  • Potentiometric probes offer indirect method of detecting the translocation of these ions
  • Increases and decreases in membrane potential-referred to as membrane hyperpolarization and depolarization, respectively-play central role in many physiological processes, including nerve-impulse propagation, muscle contraction, cell signaling and ion-channel gating

Cell Analysis, Cell Metabolism, Cell Structure, Cell Viability, Proliferation and Function, Membranes (General) and Lipids

Order Info

Shipping Condition: Room temperature



Cell Membranes & Lipids
Store at room temperature and protect from light.
Fluorescence Microscope, Microplate Reader
Room Temperature


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For Research Use Only. Not for use in diagnostic procedures.