Invitrogen™ Dextran, Oregon Green™ 488; 10,000 MW, Anionic

Rigorous methods for removing as much unconjugated dye as practical, followed by assay of dextran conjugates by thin-layer chromatography to help ensure absence of low molecular weight contaminants

Supplier:  Invitrogen™ D7170

Catalog No. D7170

Description

Labeled dextrans are hydrophilic polysaccharides most commonly used in microscopy studies to monitor cell division, track the movement of live cells, and to report the hydrodynamic properties of the cytoplasmic matrix. The labeled dextran is commonly introduced into the cells via microinjection. Dextrans are hydrophilic polysaccharides characterized by their moderate-to-high molecular weight, good water solubility, and low toxicity. They also generally exhibit low immunogeniticy. Dextrans are biologically inert due to their uncommon poly-(α-D-1,6-glucose) linkages, which render them resistant to cleavage by most endogenous cellular glycosidases.

• Label (Ex/Em): Oregon Green™ 488 (496/524)
• Size: 10,000 MW
• Charge: Anionic
• Fixable: Nonfixable
• Succinimidyl coupling of dyes to the dextran molecule, which, in most cases, results in a neutral or anionic dextran. The reaction used to produce the Rhodamine Green™ and Alexa Fluor™ 488 dextrans results in the final product being neutral, anionic, or cationic
• Common uses include neuronal tracing (anterograde and retrograde) in live cells
• Cell lineage tracing in live cells
• Neuroanatomical tracing
• Examines intercellular communications (e.g., in gap junctions, during wound healing, and during embryonic development)
• Investigates vascular permeability and blood-brain barrier integrity
• Tracks endocytosis
• Monitors acidification (some dextran-dye conjugates are pH-sensitive)
• Studies hydrodynamic properties of the cytoplasmic matrix

• Oregon Green dextrans are intrinsically anionic
• To produce more highly anionic dextrans, proprietary procedure adds negatively charged groups to dextran carriers; these products are designated polyanionic dextrans

Cell Analysis, Cell Tracing and Tracking, Cell Viability, Proliferation and Function, General Cell Tracing, Ionic Homeostasis and Signaling, Neuronal Tracing, pH Detection

Order Info

Shipping Condition: Room temperature

Specifications

• Product Type: Dextran
• Label or Dye: Other Label(s) or Dye(s)
• Excitation/Emission: 496/524 nm

• Content And Storage: Store in freezer (-5 to -30°C) and protect from light.
• Shipping Condition: Room Temperature
• Product Line: Oregon Green™

For Research Use Only. Not for use in diagnostic procedures.