Lyle and Louise: An Identity Crisis™ DNA Typing
Introduces gel electrophoresis, Polymerase Chain Reaction (PCR) and Short Tandem Repeat (STR) DNA analysis.
Supplier: Crosscutting Concepts LLC VXH10038
Includes: Six pre-melted agarose gels, one BlueVis staining solution, two BlueVis gel buffers, six sets of DNA, six InstaStain™ Methylene Blue sheets and fully illustrated teacher's manual with technique background
Requires : Gel electrophoresis apparatus with power supply, microwave, glass bottle (>1L capacity), 10μL fixed volume pipetter with tips, 5mL serological pipet or 10mL graduated cylinder, distilled water, lab gloves
Two women have been identified post mortem as Louise Mondelo. Students determine who the real Louise is and answer questions about her paternity and others at the scene.
- Students pour agarose gels, load DNA, run the gel and analyze results
- Gels use nontoxic BlueVis™ buffer/stain solution formulated by Vandalia Science Education
- DNA can be seen as the gel runs, which provides a great visual aid to show DNA migration
- No need for hazardous stains, UV equipment or time-consuming staining procedures
- Class size: six groups of up to five students per group
|Meets National Science Education Standards|
|For introducing gel electrophoresis, polymerase chain reaction and short tandem repeat dna analysis|
|Mystery Of Lyle And Louise: An Identity Crisis™ Dna Typing Forensic Science Curriculum Kit|
|9 to Undergraduate|
|Six pre-melted agarose gels, one bluevis staining solution, two bluevis gel buffers, six sets of dna, six instastain™ methylene blue sheets, and fully illustrated teacher's manual with technique background|
|Gel electrophoresis apparatus with power supply, microwave, glass bottle, 10μL fixed volume pipetter with tips, 5mL serological pipet or 10mL graduated cylinder, distilled water, lab gloves|
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