Kemtec™ Separation of Plant Pigments Using Gel Filtration Kit

Students learn how to separate pigments in plants.

Supplier:  Aqua Phoenix Scientific 8502

Catalog No. S25828

Includes: Six PD-10 columns, filter paper, ethyl alcohol solution, Sephadex G-25, MSDS, methods of assessment manual, student data sheets and instructor manual

Description

Students pack Sephadex G-25 chromatographic column and calibrate it, then see a separation of anthocyanin, carotene, chlorophyll and xanthophyll from a mixture of these compounds.This separation is due to differences in molecular structures of these aromatic and cyclic compounds. The greater the number of rings in the pigments, the greater the affinity of the pigments for the gel granules.

• Students separate pigments found in plants based on differences in molecular charge and structure
• Students pack their own Sephadex G-25 chromatographic columns
• Separation of anthocyanin, carotene, chlorophyll and exanthophyll from a mixture of plant pigments can be observed
• Columns may be re-used
• For grades 10 to 14
• Class size: 24 students

Specifications

• Product Type: Kemtec™ Gel Filtration of Plant Pigment Kit
• For Use With (Application): Allows the students to take advantage of differences in molecular charge and structure in order to bring about a separation of pigments found in plants
• Requires: Beet, spinach and coleus leaves, balance, lab glassware and supplies

• Grade: 10 to 14
• Includes: Student manual, material safety data sheets, student data sheets, ethyl alcohol solution, sephadex g-25, filter paper, six pd-10 columns, instructor manual
• Class Size: 24 Students