Invitrogen™ Dynabeads™ SILANE Viral NA Kit

For sensitive, highly predictable and consistent extraction and isolation of viral DNA and RNA from human serum/plasma samples, following a simple separation protocol.

Supplier:  Invitrogen™ 37011D

Catalog No. 37-011-D

Includes: Dynabeads MyOne; SILANE (5mL, 40mg/mL, see Cat. no. 370-02D for further characteristics), binding/washing/elution buffers and a protocol optimized for isolation of viral DNA/RNA. The kit content is sufficient for 96 isolations.

Description

For sensitive, highly predictable and consistent extraction and isolation of viral DNA and RNA from human serum/plasma samples, following a simple separation protocol. The 1 μm Dynabeads have an optimized silica-like surface chemistry, and a high specific surface area, providing efficient kinetics and a high sensitivity. Their uniform size and surface area ensure reproducible results.

A higher sensitivity and improved performance is achieved with Dynabeads™ compared to magnetic particles from alternative suppliers. Other particles often have a random size range distribution, surface area and binding capacity. This could compromise the reproducibility of your results. Dynabeads™ hold reputable Dynal high standards with respect to within- and between-lot reproducibility and automation ability.

Advantages
• Isolates viral DNA and viral RNA
• High sensitivity, allowing for a low detection limit
• Effective isolation of nucleic acids even from challenging viruses (e.g. HBV)
• Can detect HBV DNA in a 200 μl serum sample containing only 40 infectious units (using 2 mg (50 μl) Dynabeads™)
• Quicker than spin-columns
• Cost-effective and performs to the same level as spin-columns
• Automation-friendly (slow sedimentation rate + high magnetic mobility)

Applications
• Isolation of viral nucleic acids (DNA/RNA)
• From human serum/plasma samples
• Well suited for automated assays
• Separate kit available for the isolation of genomic DNA (Cat. no. 370-12D)

Additional Info
Validation and customization (bead, buffer, protocol or format) can be made available on a custom OEM basis. For further information, please contact us at: ivd@invitrogen.com

Order Info

Shipping Condition: Room Temperature

Specifications

• Content And Storage: • 5 mL Dynabeads MyOne SILANE; 4°C
  • 30 mL Lysis/binding Buffer; 4°C
  • 2 x 45 mL Washing Buffer 1; 4°C
  • 30 mL Washing Buffer 2; 4°C
  • 10 mL Elution Buffer; 4°C
  
  ,

  The kit contains: Dynabeads™ MyOne™ SILANE (5 mL, 40 mg/mL, see Cat. No. 370-02D for further characteristics), binding/washing/elution buffers and a protocol optimized for isolation of viral DNA/RNA. The kit content is sufficient for 96 isolations.
  
  Store at: 2°C to 8°C.

• Sample Type: Plasma, Serum
• Final Product Type: Viral DNA and RNA
• For Use With (Application): RT-PCR, qPCR, cDNA library construction, NGS, microarray analysis, blot hybridization, Northern/Southern blotting, in vitro translation, nuclease protection assays, nucleic acid labeling, hybridization
• Quantity: 96 Preps
• Size: 1 μm
• Test Time: Up to 45 min.

• Isolation Technology: Magnetic Bead
• Elution Volume: 100 μL
• For Use With (Equipment): Automated Liquid Handling Systems
• High-throughput Compatibility: High-throughput Compatible
• Shipping Condition: Room Temperature
• Target: Viral DNA/RNA
• Starting Material Amount: Serum: 200 μL
  Plasma: 200 μL

For Research Use Only. Not for use in diagnostic procedures.