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Invitrogen™ 5-(3-Aminoallyl)-dUTP (50 mM)

When incorporated into DNA, Ambion 5-(3-aminoallyl)-dUTP provides a reactive group for addition of other chemical groups.

Supplier:  Invitrogen™ AM8439

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Catalog No. AM8439


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Description

Description

When incorporated into DNA, Ambion 5-(3-aminoallyl)-dUTP provides a reactive group for addition of other chemical groups. The modified dUTP is readily incorporated by reverse transcriptase. The aminoallyl modification enables downstream reaction with amine-reactive compounds such as N-hydroxysuccinimide (NHS) esters; thus aminoallyl-modified cDNA can be labeled with any amine-reactive group moiety.

Use of this modified nucleotide
Use 5-(3-aminoallyl)-dUTP as recommended in your protocol for reverse transcription. Substitution of 30–80% of the dTTP with 5-(3-aminoallyl)-dUTP may be useful, but the exact proportion will depend on experimental goals.

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Shipping Conditions: Dry Ice

TRUSTED_SUSTAINABILITY
Specifications

Specifications

5-(3-Aminoallyl)-dUTP, 50 mM (50 μL)

Store at –70°C.
Aminoallyl
Nucleotides
50 μL
Nucleic Acid Labeling
Liquid
Ambion™
HPLC
Dry Ice
50 mM
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