Invitrogen™ AccuPrime™ Pfx DNA Polymerase

Provides higher fidelity than Pfu DNA polymerase

Supplier:  Invitrogen™ 12344032

Catalog No. 12-344-032

Includes: AccuPrime Pfx DNA Polymerase (100μL, at 2.5U/μL) is provided with 10X AccuPrime reaction mix (1mL) that contains dNTPs and a separate vial (1mL) of 50mM MgSO₄. Reagents are provided for 200 amplification reactions of 50μL each.

Description

AccuPrime Pfx DNA Polymerase is ideal for high-fidelity amplification of DNA fragments for downstream applications such as cloning and mutagenesis. High fidelity is provided by a proprietary enzyme preparation containing recombinant DNA polymerase from Thermococcusspecies KODwith proofreading (3'-5' exonuclease) activity. Platinumanti-PfxDNA polymerase antibodies inhibit polymerase activity, providing hot-start capabilities for improved PCR specificity. Thermostable AccuPrime accessory proteins enhance specific primer-template hybridization during every cycle of PCR, increasing specificity, yield, and robustness over PlatinumPfx alone.

• Achieve greater than 26 times higher fidelity than Taq DNA polymerase
• Amplify fragments up to 12 kb
• Minimize PCR optimization
• Built-in hot start

Applications
Amplification of DNA from complex genomic, viral, and plasmid templates; and RT-PCR. AccuPrime Pfx DNA Polymerase is especially effective where very high levels of fidelity are required.

Amplification of Bisulfite-Treated DNA, High Fidelity PCR, Hot Start PCR, Methylation Analysis, PCR, PCR and Real-Time PCR, RNAi, Epigenetics and Non-Coding RNA Research

Order Info

Approved for shipment on Wet or Dry Ice

Specifications

• Content And Storage: • 1 x 500 μL AccuPrime Pfx DNA Polymerase (2.5 U/μL)
  • 5 x 1 mL 10X AccuPrime Pfx Reaction Mix
  • 2 x 1 mL 50 mM MgSO₄
  
  Store at -20°C.
• Format: Tube
• Polymerase: AccuPrime Pfx DNA Polymerase
• Exonuclease Activity: 3' - 5'
• Quantity: 1000 Reactions
• Fidelity (vs. Taq): 26 X
• No. of Reactions: 1000 Reactions
• Reaction Format: Separate Components
• Starting Material: DNA

• Detection Method: Primer-probe
• GC-Rich PCR Performance: Low
• Reaction Speed: Standard
• Product Line: AccuPrime™
• Shipping Condition: Approved for shipment on Wet or Dry Ice
• Hot Start: Built-In Hot Start
• Overhang: Blunt
• Size (Final Product): 12 kb or less
• Volume: 500 μL

For Research Use Only. Not for use in diagnostic procedures.