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Innovating Science™ AP Biology Investigation Kit No. 8: Bacterial Transformation

Students genetically engineer bacteria through transformation with a plasmid that confers antibiotic resistance.

Supplier:  Innovating Science™ IS3708

Catalog No. S07058

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  • Sterile Calcium Chloride 0.05M (30mL)
  • 16 Sterile volumetric pipets
  • Eight Pipet bulbs
  • Eight micropipets w/plunger (10μL)
  • 16 Microcentrifuge Tubes (1.5mL)
  • Eight sterile inoculating loops
  • 40 Petri dishes
  • Four Luria agar (200mL)
  • One Luria broth tube (9mL)
Perishable materials are supplied via coupon redeemable by fax, phone or e-mail, and include:
  • One E. Coli culture (freeze dried)
  • Two bottles 0.02g Ampicillin
  • 1000ng pUC 19 Plasmid
  • One Tryptic Soy Broth tube (9mL)
  • One Tryptic Soy Agar slant (6mL)
  • One sterile, disposable pipet




  • Efficiency of the procedure is determined by examining the number of resistant bacteria
  • Includes teachers manual and student study guide copymasters
  • Meets AP Science Practices 1, 3, 5, 6, and 7, and Big Idea 3
  • For eight lab groups


Recommended: Incubator (37°C). Requires: Large beaker, microwave (not necessary if melting agar in a waterbath), microfuge tube racks, hot plate or waterbath, markers, and container of ice.



Meets AP science practices 1, 3, 5, 6, and 7 and Big Idea 3
13 to 20
30ml 0.05m sterile calcium chloride, 16 sterile volumetric pipets, 8 pipet bulbs, 8 x 10μl micropipets with plunger, 16 x 1.5ml microcentrifuge tubes, 8 sterile inoculating loops, 40 petri dishes, 4 x 200ml luria agar, 9ml luria broth tube and coupon for perishable supplies
12.125 x 9.25 x 6.5 in.
AP™ Biology Investigation Kit #8: Biotechnology: Bacterial Transformation
7 to 14
Students genetically engineer bacteria through transformation with a plasmid that confers antibiotic resistance
8 Lab Groups


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