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Edvotek™ In Search of the Cholesterol Gene
This experiment includes reagents for the colorimetric enzymatic reaction which is the basis of the clinical cholesterol test. In addition, using agarose gel electrophoresis, students will analyze a simulated genetic screening for a disease.
Supplier: Edvotek™ 316
Includes: instructions, cholesterol standard solution, standard DNA markers, control samples, simulated patient serum samples and DNA samples, cholesterol oxidase enzyme, potassium iodide, color enhancer & color developer, agarose, electrophoresis buffer, InstaStain™ Ethidium Bromide
Requires :
- Horizontal gel electrophoresis apparatus
- Hotplate, micropipets, balance, waterbath, and spectrophotometer
- 250mL Flasks
- UV transilluminator
- All necessary safety equipment
- Distilled or deionized water
Description
Coronary heart disease and stroke are major causes of death in the Western world. Elevated blood cholesterol levels are a serious risk factor for both conditions. The genetic disease familial hypercholesterolemia (FH) causes an increase in blood levels of the 'bad' form of cholesterol, low-density lipoprotein (LDL). In untreated patients with the mutant FH gene, the condition can cause premature death. This experiment includes reagents for the colorimetric enzymatic reaction which is the basis of the clinical cholesterol test. In addition, using agarose gel electrophoresis, students will analyze a simulated genetic screening for a disease.Specifications
| Agarose gel electrophoresis | |
| Horizontal gel electrophoresis apparatus, power supply, automatic micropipette with tips, balance, microwave oven or hot plate, incubation oven or water bath, spectrophotometer and cuvettes, UV transilluminator, pipet pumps or bulbs, lab glassware, large weigh boats | |
| Some components require freezer and refrigerator storage |
| Instructions, cholesterol standard solution, standard DNA markers, control samples, simulated patient serum samples and DNA samples, cholesterol oxidase enzyme, potassium iodide, color enhancer and color developer, agarose, electrophoresis buffer, InstaStain™ ethidium bromide | |
| Complete in 2 hours 15 minutes | |
| 10 Lab Groups |
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