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Cytiva IMAC Sepharose™ High Performance Media
For optimizing purification of histidine-tagged proteins using Immobilized Metal Affinity Chromatography (IMAC) when Ni2+ is not the best choice of metal ion
Supplier: Cytiva 17092007
Description
- 34μm beads of highly cross-linked 6% agarose, to which a chelating group has been covalently coupled
- Charge with transition metal of choice for optimized selectivity
- Small bead size for high performance purification
- High binding capacity: ≥40mg histidine-tagged protein/mL medium when charged with Ni2+
- Allows more sample to be applied; a more concentrated target protein is obtained more at a lower cost/mg and in less time
- Stable in pH range 3-12 (long term), and in all commonly used buffers
Avoid use with chelating agents, e.g., EDTA, EGTA, or citrate.
Specifications
| <300 cm/hr. | |
| 34 μm | |
| 4°C to 30°C | |
| Purification of Polyhistidine tagged Proteins |
| 3 to 12 | |
| IMAC Sepharose HP | |
| 100 mL |
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