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Cytiva Hot Start Master Mix

A ready-to-use 2X mastermix providing robust and reliable performance for demanding PCR applications in which high specificity and high sensitivity are essential to success

Supplier:  Cytiva 25150001

Catalog No. 46-001-001

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  • Convenient, ready-to-use mix saves time and reduces experimental variability
  • For any standard PCR reaction, such as multiplex PCR, and reactions exhibiting nonspecific amplification or primer-dimer formation
  • Primer sequestration effectively reduces any nonspecific priming or primer-dimer formation
  • Recombinant activator protein avoids the risk of contamination from a mammalian source associated with hot start antibodies
  • Avoids the risk of contamination from a mammalian source associated with hot start antibodies
  • Quick hot start activation preserves sample integrity by avoiding extensive precycling heat treatment



2X Concentrate solution of 20mM Tris HCl (pH 8.6), 100mM KCl, 3mM MgCl2, 0.4mM dNTPs (dATP, dCTP, dGTP, dTTP), 50U/mL Taq DNA Polymerase, hot start activator protein, stabilizers. Store at -20°C
100 Reactions
Hot Start Master Mix is a 2X premixed formulation that can effectively reduce nonspecific priming and primer-dimer formation during PCR.
Standard PCR


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