IgG2a, κ Isotype Control Mouse, Brilliant Violet 750, Clone: G155-178, BD

Mouse Monoclonal Antibody

Supplier:  BD Biosciences 566368

Catalog No. BDB566368

Description

The G155-178 clone has an unknown specificity. Trinitrophenol (TNP), the immunogen, is a hapten not expressed on human, mouse, rat or non-human primate cells. In the absence of specific binding, this antibody may bind non-specifically to immunoglobulin Fc receptors. The immunoglobulin secreted by the G155-178 hybridoma was selected as a mouse IgG2a, κ isotype control following screening for low background binding on a variety of mouse and human tissues.

The antibody was conjugated to BD Horizon BV750 which is part of the BD Horizon Brilliant™ Violet family of dyes. This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405nm and an acceptor dye with an Em Max at 750nm. BD Horizon Brilliant BV750 can be excited by the violet laser (405 nm) and detected with a 750/30 nm filter with a 740 nm long pass. Due to spectral differences between labeled cells and beads, using BD™ CompBeads can result in incorrect spillover values when used with BD Horizon BV750 reagents. Therefore, the use of BD CompBeads or BD CompBeads Plus to determine spillover values for these reagents is not recommended. BV750

Specifications

• Antigen: Mouse IgG2a, κ Isotype Control
• Clone: G155-178
• Conjugate: Brilliant Violet 750
• Gene Alias: IgG2a kappa Isotype Control
• Immunogen: TNP-keyhole limpet hemocyanin
• Regulatory Status: RUO
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
• Isotype: IgG2a κ

• Classification: Monoclonal
• Concentration: 0.2mg/mL
• Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
• Host Species: Mouse
• Quantity: 50 μg
• Primary or Secondary: Primary
• Form: Liquid