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MilliporeSigma™ Human Dopaminergic Neurogenesis Kit
Contains necessary growth factors and complete differentiation media needed to differentiate human ES/iPS cells into dopaminergic neurons using 21 day differentiation protocol. MilliporeSigma™; Human Dopaminergic Neurogenesis Kit has been validated with 4 human ES and iPS lines for efficient dopaminergic neuronal differentiation.
Supplier: MilliporeSigma™ SCR135
Description
- Contains collection of five validated growth factors that are routinely used to induce differentiation of human pluripotent ES/iPS cells to dopaminergic neurons
- Has high affinity antibody marker, anti-tyrosine hydroxylase, to aid in quantifying percentage yields from differentiation experiments
- H1: 30% TH positive neurons
- H9: 50-70% TH positive neurons
- Two Human iPS cell lines: ∼ 50% TH positive neurons
- Components:
- 2 vials of human recombinant Sonic Hedgehog (Shh) (5ug/vial)
- 1 vial of human recombinant FGF-8 (5ug/vial)
- 1 vial of human recombinant BDNF (2ug/vial)
- 1 vial of human recombinant GDNF (2ug/vial)
- 1 vial of human recombinant TGF-b-III (2ug/vial)
- 1 vial of anti-tyrosine hydroxylase, rabbit polyclonal (20uL)
Specifications
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Cell Differentiation, Stem Cell Culture, Cell Culture, Neurotoxicity, Neuroscience | |
RUO |
Neurogenesis Kit | |
225mL Media and Growth Factor Kit |
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