Cytiva HiTrap™ Protein G HP Prepacked Columns

Use for fast, easy separations of IgG from different species and subclasses. Cytiva Life Sciences™ HiTrap™ Protein G HP Prepacked Columns are prepacked with Protein G Sepharose™.

Catalog Number	Mfr. No.	Binding Capacity	Diameter (English) Inner Column	Flow Rate	Volume (Metric) Bed
45-000-053	Cytiva 17040401	25 mg Human IgG/Column	0.27 in.	<4 mL/min.	1 mL
45-000-055	Cytiva 17040501	125 mg Human IgG/Column	0.62 in.	>20 mL/min.	5 mL
45-000-054	Cytiva 17040403	25 mg Human IgG/Column	0.27 in.	>4 mL/min.	1 mL
45-000-056	Cytiva 17040503	125 mg Human IgG/Column	0.62 in.	>20 mL/min.	5 mL
45-000-504	Cytiva 29048581	25 mg Human IgG/Column	0.27 in.	<4 mL/min.	1 mL

Description

• Prepacked with Protein G Sepharose High Performance
• Binding capacity: 25mg human IgG/column
• Maximum pressure: 3 bar
• Column material: polypropylene
• For use with syringe, peristaltic pump, or chromatography system
• 1 and 5mL columns

Media

• Ligand: Recombinant protein G lacking albumin-binding region
• Matrix: 6% cross-linked agarose
• Average particle size: 34μm
• pH stability working range: 3 to 9

1mL columns

• Bed dimensions: 7 x 25mm
• Flow velocity: <4mL/min.

5mL columns

• Bed dimensions: 16 x 25mm
• Flow velocity: <20mL/min.

Specifications

• Content And Storage: 4°C to 30°C
• Description: HiTrap Protein G HP are prepacked columns for general-purpose preparative purification of monoclonal and polyclonal antibodies from most species including rat.
• For Use With (Application): Designed for use with a syringe, peristaltic pump, or chromatography system
• Storage Buffer: 20% Ethanol