Cytiva HiTrap™ IMAC Columns

For optimizing purification of histidine-tagged proteins when Ni ⁺² is not the best choice of metal ion

Includes: Instructions, plus a variety of connectors for syringe, peristaltic pump, and chromatography systems

Catalog Number	Mfr. No.	Diameter (English) Inner	Quantity	Particle Size
45-000-163	Cytiva 17092003	0.27 in.	5 x 1 mL	34 μm
45-000-164	Cytiva 17092005	0.62 in.	5 x 5 mL	34 μm
45-000-167	Cytiva 17092102	0.27 in.	5 x 1 mL	90 μm
45-000-168	Cytiva 17092104	0.62 in.	5 x 5 mL	90 μm

Description

• Conveniently charge with your metal of choice
• Can be used with a syringe, peristaltic pump, or liquid chromatography system
• Available prepacked with IMAC Sepharose™ High Performance (HP), with small bead size for high performance purification, or IMAC Sepharose 6 Fast Flow (FF), with larger bead size for easy scaleup
• High binding capacity: ≥40mg histidine-taged protein/mL medium (protein/metal ion dependent)
• Stable in the presence of all commonly used buffers
• Available in 1mL and 5mL sizes

Specifications

• Flow Rate: <4 mL/min.
• Material: Polypropylene
• pH Range: 3 to 12
• For Use With (Application): For optimizing purification of His-tagged Proteins

• For Use With (Equipment): Syringe, peristaltic pump or liquid chromato graphy system
• Max. Pressure: 42 psi
• Content And Storage: 4°C to 30°C