Cytiva HiTrap™ AVB Sepharose™ Columns

Enables purification of adeno associated viruses (AAV) in one step, with high purity and excellent yields. Cytiva HiTrap™ AVB Sepharose™ Columns are pre-packed with affinity chromatography medium (resin) for simple operation with a syringe, pump or chromatographic system.

Catalog Number	Mfr. No.	Quantity	Diameter (Metric) Inner
45-200-59	Cytiva 28411211	5 x 1 mL	7 mm
45-200-60	Cytiva 28411212	1 x 5 mL	16 mm

Description

AVB Sepharose High Performance is an affinity resin designed for the purification of adeno associated virus (AAV). It enables purification of adeno associated viruses in one step with high purity and excellent yields.

• One step purification of adeno associated viruses
• Affinity resin with high selectivity and low background binding
• Large surface area to give enhanced capacity
• Prepacked HiTrap columns for simple operation with a syringe, pump, or chromatographic system

AVB Sepharose High Performance has proven affinity for adeno associated viruses from subclasses 1, 2, 3, and 5.

• It is expected to have affinity for most of the other adeno associated virus subclasses
• The ligand is a 14 kD fragment from a single chain antibody, expressed in yeast

Specifications

• For Use With (Application): One Step Purification of Adeno Associated Viruses
• Storage Requirements: Store prepacked column at 4°C to 30°C in 20% Ethanol and Media at 4°C to 8°C in 20% Ethanol.
• Product Type: HiTrap AVB Sepharose HP Column
• Particle Size: 24 to 44 μm
• Material: Polypropylene
• Matrix: Cross-linked agarose
• Ligand Type: 14 kD recombinant protein

• Stability: Stable to all commonly used aqueous buffers
• Binding Capacity: >10▿12 genecopies/mL Chromatography Mediμm
• Flow Rate: <150 cm/hr
• Height (Metric) Bed: 25 mm
• Max. Pressure: 70 psi (5 bar)
• pH Range: 3 to 10