Goat anti-Mouse IgG (H+L) Secondary Antibody, DyLight™ 405, Invitrogen™

Goat Polyclonal Secondary Antibody

Supplier:  Thermo Scientific 35501BID

Catalog No. PI35501

Description

Product # 35501 has been successfully used in Western blot, IF, ICC, IHC, IP and FACS applications. Product # 35501 reacts with the heavy chains of mouse IgG and with the light chains common to most mouse immunoglobulins, but does not react against non-immunoglobulin serum proteins. However, this antibody may cross-react with immunoglobulins from other species. Store product protected from light at 4°C until opened. To extend the shelf-life of this product, add an equal volume of glycerol to make a final concentration of approximately 50% glycerol and store at -20°C. DyLight 405 Amax= 400 nm; Emax= 420 nm. Mole Dye/Mole Protein Ratio is lot-dependent.

Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

Specifications

• Antigen: Mouse IgG (H+L)
• Classification: Polyclonal
• Conjugate: DyLight 405
• Host Species: Goat
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Target Species: Mouse
• Form: Liquid

• Applications: Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot
• Concentration: 1 mg/mL
• Formulation: PBS with 1% BSA and 0.02% sodium azide; pH 7.2
• Immunogen: Purified Mouse IgG, whole molecule
• Quantity: 1 mL
• Primary or Secondary: Secondary
• Content And Storage: 4° C
• Isotype: IgG