R&D Systems™ FlowX Rat Regulatory T Cell Multi-Color Flow Kit

R&D Systems™ FlowX Rat Regulatory T Cell Multi-Color Flow Kit is designed for the flow cytometric analysis of Treg cells using three fluorochrome-conjugated antibodies.

Supplier:  R&D Systems™ FMC015B

Catalog No. FMC015B

Description

• This kit contains three conjugated antibodies (and a mouse IgG1 isotype control) that can be used for single-step staining of rat regulatory Treg cells (1-9).
• Positive Markers:
  • 125μL of FoxP3 Alexa Fluor™ 647 Mouse IgG1 (Clone 376209)
  • 250μL of CD25/IL-2 Rα-PE Mouse IgG1 (Clone 745520)
  • 250μL of CD4-Fluorescein Mouse IgG2A (Clone OX-38)
• Isotype Control:
  
  • 250μL of Mouse IgG1 Alexa Fluor™ 647 Mouse IgG1 (Clone 11711)
• FoxP3/Transcription Factor Fixation Concentrate (4X):
  • 8mL of a formaldehyde solution.
• FoxP3/Transcription Factor Fixation Diluent:
  • 25mL of a buffered detergent.
• FoxP3/Transcription Factor Permeabilization & Wash Buffer (10X):
  • 25mL of a buffered protein base with detergent and preservatives. May contain a precipitate but will not affect product performance.
• Staining Buffer (1X):
  • 50mL of a 1X staining buffer.
• Some components in this kit contain formaldehyde which is a suspected carcinogen.
• Avoid contact with skin, eyes, and mucous membranes, and avoid inhaling fumes.
• In case of contact, wash immediately with water and seek medical advice.
• Some components in this kit contain sodium azide which may react with lead and copper plumbing to form explosive metallic azides.
• Flush with large volumes of water during disposal.

Specifications

• No. of Tests: 25
• Content And Storage: Store the unopened product at 2 - 8° C. Do not use past expiration date.