Fisher BioReagents™ SurePrep™ RNA/Protein Purification Kit

Rapid method for the isolation and purification of total RNA and proteins sequentially from a single sample using the same column in less than 20 minutes

Catalog No. BP280650

Includes: Lysis solution (40mL); Nucleic acid wash solution (22mL); Nucleic acid elution buffer (15mL); Protein column regeneration buffer (30mL); Protein column activation and wash buffer (60mL); Protein pH binding buffer (4mL); Protein elution buffer (8mL); Enzyme incubation buffer (6mL); Protein neutralizer (2mL); Protein loading dye (2mL); Spin colulmns (50); Collection tubes (150); Product insert (1)

Description

• Fast and easy processing using rapid spin-column format
• All columns for RNA purification and protein purification provided
• Sequentially isolate high quality RNA and proteins from a single lysate – no need to split the lysate
• Isolate all sizes of RNA, from large mRNA and rRNA down to microRNA (miRNA)
• No phenol or chloroform extractions
• Purify total RNA and proteins from a variety of biological sources such as cultured animal cells, tissue samples, blood, bacterica, yeast, fungi or plants

Applications:

Purified total RNA and protein from same biological sample for studies of gene expression including gene silencing experiments, mRNA knockdowns, or experiments correlating RNA and protein expression levels. RNA qualified for use in real time PCR, reverse transcription PCR, Northern blotting, RNase protection and expression array assays. Proteins suitable for use in SDS-PAGE analysis and Western blots.

Specifications

• Format: Spin Column
• For Use With (Application): Purified total RNA and Protein from same biological sample for studies of gene expression including gene silencing experiments, mRNA knockdowns or experiments correlating RNA and Protein expression levels. RNA qualified for use in real time PCR, reverse transcription PCR, northern blotting, RNase protection and expression array assays. Proteins suitable for use in SDS-PAGE analysis and Western blots
• Includes: Lysis solution 40mL, Nucleic Acid wash solution 22mL, Nucleic Acid Elution Buffer 15mL, Protein column regeneration Buffer 30mL, Protein column activation and Wash Buffer 60mL, Protein pH Binding Buffer 4mL, Protein Elution Buffer 8mL, Enzyme incubation Buffer 6mL, Protein neutralizer 2mL, Protein loading dye 2mL, spin columns 50, collection tubes 150, product insert 1
• Quantity: 50 Preps