Fisher BioReagents™ SurePrep™ Nuclear or Cytoplasmic RNA Purification Kit

Rapid method for the isolation and purification of both nuclear and cytoplasmic RNA from cultured animal cells and animal tissue samples

Catalog No. BP280550

Includes: Lysis solution (20mL); Wash solution (22mL); Binding solution (25mL); RNA elution solution (6mL); Spin columns (50); Collection tubes (50); Elution tubes (1.7mL) (50); Product insert (1)

Description

Rapid method for the isolation and purification of both nuclear and cytoplasmic RNA from cultured animal cells and animal tissue samples

• Fast and easy processing using rapid spin-column format
• High quality intact RNA can be used in various downstream applications
• Enrich for cytoplasmic or nuclear RNA depending on downstream application
• No genomic DNA contamination in the cytoplasmic fraction
• No phenol or chloroform extractions
• Isolate all sizes of RNA from each fraction, including all small RNA molecules (>200 nt)
• Kit provides sufficient reagents to perform either 50 cytoplasmic RNA preparations or 25 cytoplasmic and nuclear preparations

Applications:

Purification of Nuclear and Cytoplasmic RNA suitable for real-time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays.

Specifications

• Format: Spin Column
• Includes: Lysis solution 20mL, wash solution 22mL, binding solution 25mL, RNA elution solution 6mL, spin columns 50, collection tubes 50, elution tubes 1.7mL 50, product insert 1 50μg Column Binding Capacity, 600μL Max. column lo
• Yield: 35μg Grape leaves (50mg), 32μg plum leaves (50mg), 60μg tobacco leaves (50mg), 60μg tomato leaves (50mg)

• For Use With (Application): RT-PCR, reverse transcription PCR, northern blotting, RNase protection and primer extension and expression array assays
• Quantity: 75 Preps