Edvotek™ Fermentation and Bioprocessing of Chromogenic Proteins
Designed to introduce the basic concepts of fermentation and bioprocessing through the production of GFP protein in a small-scale fermentor. Edvotek™ Fermentation and Bioprocessing of GFP Experiment Kit is for 10 lab groups.
Supplier: Edvotek™ 305
DescriptionBioprocessing is the production and isolation of desired products from living cells. In this introduction to bioprocessing, students will use small-scale fermenters to produce chromogenic proteins using Escherichia coli. Protein extracts will then be separated using column chromatography to analyze the success of the fermentation process. Finally, the protein solutions will be examined by SDS polyacrylamide gel electrophoresis to determine the purity of the chromogenic proteins.
|Fermentation and Bioprocessing of GFP Experiment Kit|
|Complete in 5-6 hours|
|10 Lab Groups|
|Instructions, BactoBeads™ with GFP plasmid, LB Growth Media, Growth Additive, Ampicillin, IPTG, GFP Extraction Buffer, Microcentrifuge Tubes, Syringe, 15 ml Centrifuge tubes, transfer pipettes, loops.|
|Refrigerator and Freezer Storage (some components)|
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