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Thermo Scientific™ RevertAid H Minus First Strand cDNA Synthesis Kit

Thermo Scientific RevertAid H Minus First Strand cDNA Synthesis Kit is a complete system for efficient synthesis of first strand cDNA from RNA templates.

$162.00 - $657.00

Specifications

Format Kit
Reaction Speed 60 min.
Technique Reverse Transcription
Optimal Reaction Temperature 42°C to 45°C
Reverse Transcriptase RevertAid H Minus
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Catalog Number Mfr. No. No. of Reactions Price Quantity & Availability  
FERK1631
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Thermo Scientific™
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FERK1632
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Description

Description

Thermo Scientific RevertAid H Minus First Strand cDNA Synthesis Kit is a complete system for efficient synthesis of first strand cDNA from RNA templates. The kit includes RevertAid H Minus Reverse Transcriptase, which has a point mutation that completely eliminates RNase H activity. Therefore, it does not degrade RNA in RNA-DNA hybrids during synthesis of the first strand cDNA.

Features of the RevertAid H Minus First Strand cDNA Synthesis Kit include:
• High yields of full-length first-strand cDNA up to 13 kb
• Increased reaction temperatures of 42–55°C
• Supplied with the recombinant RiboLock RNase Inhibitor
• Complete kit includes oligo(dT)18 and random hexamer primers

Applications
• First strand cDNA synthesis for RT-PCR and RT-qPCR
• Construction of full length cDNA libraries
• Antisense RNA synthesis

Additional Features
The recombinant RiboLock RNase Inhibitor, supplied with the kit, effectively protects RNA template from degradation. It is fully compatible with the reverse transcription reaction, as it maintains activity at temperatures up to 55°C. The kit is supplied with both oligo(dT)18 and random hexamer primers. The oligo(dT)18 primer anneals selectively to the poly(A) tail of mRNA. Random hexamer primers do not require the presence of poly(A), therefore, they can be used for transcription of the 5’-end regions of mRNA or for cDNA synthesis using RNA without the poly(A) tail, e.g., microRNAs. Gene-specific primers may also be used with the kit. The first strand cDNA can be directly used as a template in PCR, real-time PCR, or in second strand cDNA synthesis.

Specifications

Specifications

Kit
Reverse Transcription
RevertAid H Minus
Dry Ice
First-Strand cDNA
Reverse Transcription
RNA
60 min.
42°C to 45°C
Reduced
Real Time PCR (qPCR), RT-PCR
Separate components
Up to 13 kb
SDS
Documents

Documents

Product Certifications

For Research Use Only. Not for use in diagnostic procedures.

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