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Thermo Scientific™ Maxima SYBR Green/ROX qPCR Master Mix (2X)
GSA_VA

Ready-to-use solution optimized for qPCR and 2-step RT-qPCR.

Supplier:  Thermo Scientific™ K0221

 View more versions of this product

Catalog No. FERK0221


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Description

Description

The master mixes include Maxima Hot Start Taq DNA Polymerase and dNTPs in an optimized PCR buffer. Only template and primers need to be added. The SYBR Green I intercalating dye allows for DNA detection and analysis without using sequence-specific probes.
Maxima Hot Start Taq DNA Polymerase in combination with an optimized buffer ensures PCR specificity and sensitivity. dUTP is included in the mix for optional carry-over contamination control using uracil DNA glycosylase (UDG). The use of Maxima SYBR Green qPCR Master Mixes in real-time PCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral, and cDNA templates. Maxima SYBR Green qPCR Master Mixes are compatible with most real-time thermal cyclers.

  • Specificity - Maxima Hot Start Taq DNA Polymerase and the optimized buffer eliminate non-specific amplification and formation of primer dimers
  • Sensitivity - detects low copy number targets
  • Wide linear range - accurate quantification across 9 orders of magnitude
  • Reproducibility and convenience - ready-to-use 2X master mix

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Specifications

Specifications

Contains:
• 2 x 1.25 mL Maxima SYBR Green/ROX qPCR Master Mix (2X) containing Maxima Hot Start Taq DNA Polymerase, dNTPs (also dUTP) and SYBR Green I in an optimized PCR buffer with ROX passive reference dye; sufficient for 200 x 25 µL reactions.
• 2 x 1.25 mL Nuclease-Free Water

Store at -20°C.
SYBR
High
qPCR
Standard
2 x 1.25 mL
Maxima
200 Reactions of 25 μL
2X
Tube
200 reactions
Taq DNA Polymerase
DNA, dsDNA
7000 System, 7300 System, 7700 System, 7900HT System, StepOnePlus™ System, StepOne™ System
Real Time PCR SYBR Master Mix
Gene Expression
SDS
Documents

Documents

Product Certifications
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Promotions

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For Research Use Only. Not for use in diagnostic procedures.