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Thermo Scientific™ M13/pUC Sequencing Primer (-40), 17-mer
Thermo Scientific M13/pUC Sequencing Primer (-40), 17-mer is a synthetic single-stranded 17-mer oligonucleotide with free 5'- and 3'-hydroxyl ends.
Supplier: Thermo Scientific™ SO113
Description
Thermo Scientific M13/pUC Sequencing Primer (-40), 17-mer is a synthetic single-stranded 17-mer oligonucleotide with free 5'- and 3'-hydroxyl ends. This M13/pUC primer anneals to the region in the 5'-terminus of the lacZ gene. All primers are supplied as 10 μM aqueous solutions.
Applications
• Sequencing of DNA fragments inserted into the MCS within the lacZ gene of various cloning vectors, such as pUC19 (Cat. No. SM0221), pTZ19R, pTZ57R, M13mp18, and pBluescript II
• Colony screening by PCR
Primer Sequence: 5'-d(GTTTTCCCAGTCACGAC)-3'
Specifications
Sequencing Primer | |
Dry Ice | |
M13 | |
pUC19, pTZ19R, pTZ57R, M13mp18, pBluescript II | |
Liquid |
M13/pUC Sequencing Primer (-40), 17-mer, 10 μM (60 μL) Store at –20°C. |
|
10 μM | |
60 μL | |
Sequencing |
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For Research Use Only. Not for use in diagnostic procedures.