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Thermo Scientific™ Fluorescein-12-dUTP Solution (1 mM)
Thermo Scientific Fluorescein-12-dUTP (fluorescein-5(6)-carboxaminocaproyl-[5-{3-aminoallyl}-2'-deoxyuridine-5-triphosphate]) is supplied as 1 mM aqueous solution.
Supplier: Thermo Scientific™ R0101
Description
Thermo Scientific Fluorescein-12-dUTP (fluorescein-5(6)-carboxaminocaproyl-[5-{3-aminoallyl}-2'-deoxyuridine-5-triphosphate]) is supplied as 1 mM aqueous solution.
Fluorescein-12-dUTP can be enzymatically incorporated into DNA with:
• Reverse transcriptases
• Taq DNA polymerase (Cat. No. EP0072)
• phi29 DNA Polymerase (EP0091)
• Klenow Fragment, exo- (EP0421)
• Klenow Fragment (EP0051)
• DNA Polymerase I (EP0041)
Applications
• Enzymatic non-radioactive labeling of DNA during cDNA synthesis
• PCR
• Nick-translation
• Random primed labeling
• Primer extension
Specifications
Fluorescein-12-dUTP Solution, 1 mM (25 μL) Store at –20°C. |
|
DNA Polymerase | |
Fluorescein (FITC) | |
Dry Ice | |
1 mM |
Liquid | |
Direct Labeling | |
25 μL | |
cDNA Synthesis, Non-Radioactive Labeling |
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For Research Use Only. Not for use in diagnostic procedures.