Thermo Scientific™ Lambda DNA (dam–, dcm–)

Generate DNA size marker fragments with this linear, double-stranded DNA, a common substrate for restriction endonucleases.

Supplier:  Thermo Scientific™ SD0021

Catalog No. FERSD0021

Description

Lambda is a temperate Escherichia coli bacteriophage. The virion DNA is linear and double-stranded (48502 nt) with 12 bp single-stranded complementary 5'-ends. After the phage particle injects its chromosome into the cell, the chromosome circularizes by end joining. In the lytic pathway, phage genes encoding replication, lysis, and virion proteins are expressed. The chromosome replicates, and the replicas are cleaved and packaged into progeny phage particles. In the lysogenic pathway, phage gene expression is repressed, and the circular chromosome inserts into the bacterial chromosome by recombination.

Highlights

Phage lambda DNA is a common substrate for restriction endonucleases and for generating DNA size marker fragments. For large scale isolation of phage DNA, cI857Sam7, a mutant carrying four known mutations, is often used. The DNA sequence used to construct a phage alpha restriction map includes these mutations. The map shows enzymes that cut lambda DNA. Enzymes produced by Thermo Scientific are shown in orange (see Figure bellow).

Lambda DNA (dam-, dcm-) is isolated from a heat-inducible lysogenic E. coli GM2163 strain.

Specifications

• Concentration: 0.3 μg/μL
• Final Product Type: DNA
• Includes: [other]
• Quantity: 500 μg

• Sample Type: DNA
• For Use With (Application): CloningNucleic Acid Gel Electrophoresis
• Product Type: Lambda DNA
• Species: Lambda phage

For Research Use Only. Not for use in diagnostic procedures.