Thermo Scientific™ Aminoallyl-dUTP Solution (50 mM)

Thermo Scientific Aminoallyl-dUTP (5-[3-aminoallyl]-2'-deoxyuridine-5'-triphosphate) is supplied as 10 mM and 50 mM aqueous solutions titrated to pH 7.0 with NaOH.

Supplier:  Thermo Scientific™ R1101

Catalog No. FERR1101

Description

Thermo Scientific Aminoallyl-dUTP (5-[3-aminoallyl]-2'-deoxyuridine-5'-triphosphate) is supplied as 10 mM and 50 mM aqueous solutions titrated to pH 7.0 with NaOH. Aminoallyl-dUTP can be enzymatically incorporated into DNA and the resulting amine-containing DNA can be subsequently labeled with any amine-reactive fluorescent dye, biotin or hapten.

Applications
• Enzymatic indirect non-radioactive labeling of DNA during cDNA synthesis
• PCR
• Nick-translation
• Random-primed labeling
• Primer extension

Aminoallyl-dUTP can be enzymatically incorporated into DNA with:
• Reverse transcriptases
• Taq DNA polymerase (Cat. No. EP0072)
• phi29 DNA Polymerase (EP0091)
• Klenow Fragment (EP0051)
• Klenow Fragment, exo- (EP0421)
• DNA Polymerase I (EP0041)

Specifications

• Content And Storage: Aminoallyl-dUTP Solution, 50 mM (50 μL)
  
  Store at –20°C.
• Polymerase: DNA Polymerase
• Label or Dye: Aminoallyl
• Shipping Condition: Dry Ice
• Concentration: 50 mM

• Form: Liquid
• Labeling Method: Direct Labeling
• Quantity: 50 μL
• For Use With (Application): Standard PCR, Gene Expression, Non-Radioactive Labeling

For Research Use Only. Not for use in diagnostic procedures.