Novex™ Endogenous Biotin-Blocking Kit
Endogenous Biotin-Blocking Kit
Supplier: Novex™ E21390
DescriptionThe Endogenous Biotin-Blocking Kit provides a method and reagents for a pretreatment that reduces or eliminates background signals when biotin-avidin or biotin-streptavidin detection systems are used to identify cellular targets.
The Endogenous Biotin-Blocking Kit offers:
Biotin occurs naturally, functioning as an enzyme cofactor in the cytosol and mitochondria of a wide variety of cell types. The Endogenous Biotin-Blocking Kit minimizes interference from the endogenous biotin found in enzymes of mammalian cells and tissues, through a two-step blocking strategy. First, an excess of unlabeled streptavidin is added to the specimen to bind endogenous biotin-rich enzymes. The streptavidin is subsequently blocked with an excess of unlabeled biotin, effectively rendering the cell or tissue sample free of available biotin-binding sites.
|Endogenous Biotin-Blocking Kit|
|Blocking⁄Background Suppression Reagent|
|Store in refrigerator at 2°C to 8°C|
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For Research Use Only. Not for use in diagnostic procedures.