MilliporeSigma™ Upstate™ Nanog Mouse monoclonal, ChIP Validated Antibody and Primer Set

Mouse Monoclonal Antibody

Supplier:  MilliporeSigma™ 17655

Catalog No. 17-655-MI

Description

Specifically detects Nanog Clone: in Human samples, and it is validated for ChIP, Immunocytochemistry, Western Blotting

NANOG (Homeobox protein NANOG) is a member of the Nanog homeobox family of DNA-binding proteins. It is expressed in embryonic stem cells and confers pluripotency on these cells. Once embryonic stem cells become differentiated, NANOG expression is suppressed.  NANOG is involved in the Hedgehog/Gli1 signaling pathway which has been implicated in the development and growth of various types of tumors.

Specifications

• Antigen: Nanog
• Classification: Monoclonal
• Formulation: Anti-Nanog (Mouse Monoclonal). One vial containing 110μL (0.7 mg/mL) purified mouse monoclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4),150mM NaCl, and 0.05% sodium azide, before the addition of 30% glycerol. Normal Mouse IgG. One vial containing 125μg of purified mouse IgG in 125μL storage buffer containing 0.1% sodium azide. ChIP Primers, miR-302. One vial containing 75μL of 5μM of each primer specific for human miR-302 cluster promoter region (chr4:113570181-113570261, hg19 build). FOR: CCC GTG GAA GCA ATC TAT TTA TTT; REV: TGT GTT TCT ATC TGG AGG AAC TCT GT
• Gene Symbols: NANOG
• Immunogen: GST-tagged recombinant protein corresponding to human NANOG.
• Quantity: 25 Assays
• Research Discipline: Epigenetics and Nuclear Function
• Gene ID (Entrez): NP_079141
• Target Species: Human
• Form: Purified

• Applications: ChIP Assay, Immunocytochemistry, Western Blot
• Conjugate: Unconjugated
• Gene Accession No.: Q9H9S0
• Host Species: Mouse
• Purification Method: Protein G purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Includes: Use ChIPAb+ Nanog ChIP Validated mouse Monoclonal for the detection of Nanog.
• Content And Storage: -20°C for one year from date of shipment; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles

For Research Use Only