MilliporeSigma™ Upstate™ Acetyl-Histone H4 (Lys16), Rabbbit Polyclonal, ChIP Validated Antibody and Primer Set

Rabbit Polyclonal Antibody

Supplier:  MilliporeSigma™ 1710101

Catalog No. 17-101-01MI

Description

Specifically detects Acetyl-Histone H4 (Lys16) Clone: in Human, Vertebrate samples, and it is validated for ChIP, Immunoprecipitation, Western Blotting

Histone H4 is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H4 is involved with the structure of the nucleosomes of the 'beads on a string' structure. Acetylation of histone H4 occurs at several different lysine positions in the histone tail and is performed by a family of enzymes known as Histone Acetyl Transferases (HATs).

Specifications

• Antigen: Acetyl-Histone H4 (Lys16)
• Classification: Polyclonal
• Formulation: Anti-Acetyl-Histone H4 (Lys16) (rabbit polyclonal). One vial containing 50μL of affinity purified rabbit polyclonal serum in buffer containing 0.1 M Tris-Glycine (pH 7.4) 150mM NaCl with 0.05% sodium azide. Normal Rabbit IgG. One vial containing 125μg of rabbit IgG in 125μL of storage buffer containing 0.05% sodium azide. ChIP Primers, RPL10 promoter. One vial containing 75μL of 5μM of each primer specific for the promoter region of human RPL10. FOR: ACC CGT CTT CGA CAG GAC T; REV: GGA ACG GAA GAC GAG AAC AG
• Gene Symbols: HIST1H4A, H4/J, HIST1H4F, HIST1H4L, H4FN, H4FA, H4FH, HIST1H4H, H4F2, H4FM, H4/A, H4FK, H4FG, HIST2H4, H4FB, H4/K, HIST1H4I, H4/N, HIST1H4B, H4FE, H4/M, H4/a, HIST1H4E, HIST4H4, HIST2H4A, H4FC, H4FI, H4/H, HIST1H4C, H4/G, HIST1H4K, H4FJ, H4FD, H4/I, H4/B, H4/D, H4/C, HIST1H4J, HIST1H4D, H4/E
• Immunogen: KLH-conjugated Histone H4 corresponding to the N-terminus region at and around acetylated Lys16.
• Quantity: 25 Assays
• Research Discipline: Epigenetics and Nuclear Function
• Gene ID (Entrez): NP_778224.1
• Includes: This ChIPAb+ Acetyl-Histone H4 (Lys16) -ChIP Validated Antibody and Primer Set conveniently includes the antibody and the specific control PCR primers.
• Content And Storage: -20°C for up to 12 months from date of receipt; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles

• Applications: ChIP Assay, Immunoprecipitation, Western Blot
• Conjugate: Unconjugated
• Gene Accession No.: P62805
• Host Species: Rabbit
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: This antibody detects histone H4 acetylated at Lys16.
• Target Species: Human, Vertebrates
• Form: Purified

For Research Use Only