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Edvotek™ In Search of the Sickle Cell Gene by Southern Blot
Use Southern blots to detect mutations while gaining insight about the effects of sickle cell anemia.
Supplier: Edvotek™ 315
Includes: instructions, Ready-to-Load™ DNA samples, agarose, electrophoresis buffer, nylon membranes, filter paper, blot stain
Requires :
- Horizontal gel electrophoresis apparatus
- Hotplate
- Micropipets
- Balance
- Water bath
- Incubation oven
- 250mL Flasks
- All necessary safety equipment
- Concentrated HCl
- NaOH
- Forceps Distilled or deionized water
Description
Southern blotting is an important technique used widely in clinical genetics and research. By transferring DNA from an agarose gel onto a membrane, the method allows you to analyze and identify the DNA bands on a gel precisely. Your students will use Southern blotting to find a point mutation in the hemoglobin gene indicating Sickle Cell Anemia.Specifications
| Southern blotting | |
| Electrophoresis apparatus, power supply, microwave or hot plate, water bath and 80°C incubation oven | |
| Some components require freezer storage |
| Instructions, Ready-to-Load™ DNA samples, agarose, electrophoresis buffer, nylon membranes, filter paper, blot stain | |
| Electrophoresis: 45 min.; Blot: overnight; Stain and Destain: 10 min. | |
| 5 Lab Groups |
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