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Edvotek™ Drosophila Genotyping Using PCR
Advanced-level experiment uses agarose gel electrophoresis to explore DNA polymorphisms.
Supplier: Edvotek 337
Includes:
- Flies
- Primers
- Buffers
- Chemicals
- Instructions and background information
- Study questions
Requires :
- Horizontal gel electrophoresis apparatus
- Thermal cycler
- Hotplate
- Micropipetter
- Balance
- Water bath
- Microcentrifuge
- 500mL flasks
- UV transilluminator
- Pipet pump or bulb
- Isopropanol
- All necessary safety equipment
- Distilled or deionized water
Description
Students will learn about DNA polymorphisms by amplifying DNA regions that vary between wild & mutant Drosophila. Amplified DNA from wild-type and white-eyed flies are separated by agarose gel electrophoresis and analyzed.Order Info
Class size: 10 groups (20 PCR reactions).
Specifications
| Instructions, PCR EdvoBeads™, primers, DNA extraction Buffer, 200bp ladder, proteinase K, drosophila (wild type and white eye), Agarose, electrophoresis Buffer, InstaStain™ Ethidium Bromide and FlashBlue™ Stain. Store at -20°C | |
| 1 Kit | |
| Genotyping | |
| 20 |
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