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Edvotek™ DNA Fingerprinting: Southern Blot Analysis Using Non-Isotopic Detection of DNA
Introduces students to non-isotopic DNA detection and Southern Blot analysis used in DNA Fingerprinting for a hypothetical paternity determination.
Supplier: Edvotek™ 311
Includes: Instructions, predigested DNA samples, buffers, NBT/BCIP tablets, streptavidin-Alkaline Phosphatase, nylon membranes, filter paper and UltraSpec-Agarose™ powder
Description
- Includes three modules: Agarose gel electrophoresis, Southern Blot transfer, and non-isotopic detection of DNA
- For five lab groups
- Time: Electrophoresis 45 min., blotting overnight and non-isotopic 3 to 4 hr.
Specifications
DNA Fingerprinting By Southern Blot | |
Instructions, predigested dna samples, buffers, nbt/bcip tablets, streptavidin alkaline phosphatase, nylon membranes, filter paper, ultraspec agarose™ powder | |
45 min. |
Southern blot analysis | |
Electrophoresis apparatus and power supply, automatic micropipet with tips, balance, microwave or hot plate, waterbath, incubation oven, pipet pumps or bulbs, pipets, floating racks for microtest tubes, lab glassware, plastic wrap | |
5 Lab Groups |
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