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Edvotek™ Determination of Protein Molecular Weight (Polyacrylamide-based)
SCI_ED

Using prestained LyphoProteins™, subunit molecular weights are determined by analysis using denaturing SDS vertical polyacrylamide gel electrophoresis.

Supplier:  Edvotek™ 153

Catalog No. S05636


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Includes: Instructions; Denatured LyphoProtein samples; Standard protein markers; Gel-loading solution; Buffer; Protein InstaStain™

Requires : Three polyacrylamide gels (12%); MV10 vertical gel electrophoresis apparatus; Power supply; Hotplate or burner; Visualization (white light); Automatic micropipet with fine tips; Microtest tube holder; Lab glassware; Methanol; Glacial acetic acid; Distilled or deionized water

Description

Description

  • Prestained proteins with unknown molecular weights are assigned molecular weights based on the relative mobility of prestained standard protein markers

 

Experiment times:

  • Electrophoresis, one hour
  • Staining, 20 minutes
  • Destaining, two hours

 

Order Info

For six groups sharing three gels.

Recommended Storage

Some components require freezer storage

Specifications

Specifications

Protein Kit
60 minutes; Staining time: 20 minutes
6 Lab Groups
Subunit molecular weights are determined by analysis using denaturing sds vertical polyacrylamide gel electrophoresis
Refrigerated. Some components require freezer storage.
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