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Edvotek™ Determination of Protein Molecular Weight (Polyacrylamide-based)
Using prestained LyphoProteins™, subunit molecular weights are determined by analysis using denaturing SDS vertical polyacrylamide gel electrophoresis.
Supplier: Edvotek™ 153
Includes: Instructions; Denatured LyphoProtein samples; Standard protein markers; Gel-loading solution; Buffer; Protein InstaStain™
Requires : Three polyacrylamide gels (12%); MV10 vertical gel electrophoresis apparatus; Power supply; Hotplate or burner; Visualization (white light); Automatic micropipet with fine tips; Microtest tube holder; Lab glassware; Methanol; Glacial acetic acid; Distilled or deionized water
Description
- Prestained proteins with unknown molecular weights are assigned molecular weights based on the relative mobility of prestained standard protein markers
Experiment times:
- Electrophoresis, one hour
- Staining, 20 minutes
- Destaining, two hours
Order Info
For six groups sharing three gels.
Recommended Storage
Some components require freezer storage
Specifications
Protein Kit | |
60 minutes; Staining time: 20 minutes | |
6 Lab Groups |
Subunit molecular weights are determined by analysis using denaturing sds vertical polyacrylamide gel electrophoresis | |
Refrigerated. Some components require freezer storage. |
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