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Investigating Synthetic Biology
SCI_ED

Cloning experiment for the classroom.

Supplier:  Edvotek 331

Catalog No. S66821


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Includes: PCR EdvoBeads™, primer mix concentrate, template DNA concentrate, universal DNA buffer, ultrapure water, DNA standard marker, 10X gel loading solution, purification and wash buffers, spin columns and reservoirs, Dryzyme™ mix, Dryzyme™ reconstitution buffer, enzyme reaction buffer, vector cut with EcoRI and HindIII, T4 DNA ligase, ReadyPour LB agar, LB medium, BactoBeads™ (Host), Calcium Chloride, competent cell solution, control DNA, recovery broth, Kanamycin, IPTG, Salacylic acid, wintergreen scent, 50X electrophoresis buffer, UltraSpec-Agarose™, SYBR™ Safe Stain, microcentrifuge and PCR tubes, 10 ml pipet, 50 ml conical tubes, sterile loops

Requires :
  • Horizontal gel electrophoresis apparatus
  • Thermal cycler
  • Water baths
  • Incubation oven
  • Hotplate
  • Micropipets
  • Balance
  • UV transilluminator
  • Microtest tubes
  • 250mL flasks
  • Ice
  • All necessary safety equipment
  • Distilled or deionized water


Description

Description

Teach your students about synthetic biology with this exciting and exclusive lab! Students use PCR to amplify the coding sequence of the BSMT1 enzyme. This interesting enzyme is responsible for the formation of methyl salicylate, a chemical with a strong ''wintergreen'' odor. The PCR product is purified, restriction digested, and inserted into a plasmid vector. The resulting recombinant DNA is then used to transform E. coli BactoBeads™. Finally, students design an experiment to express the enzyme from their transformants and perform a smell test to confirm that the bacterial factories are working!
Specifications

Specifications

Instructions, biologicals, Buffers and reagents for PCR, ligation and transformation, ReadyPour™ luria broth agar, DNA size ladder, wax beads, Agarose, electrophoresis Buffer, Gel stain. Store at -20°C
1 Kit
Standard PCR
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