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Edvotek™ Advanced Placement Laboratory Kit 2: Principles of Enzyme Catalysis

Understand enzyme catalysis and protein structure-function relationships.

Supplier:  Edvotek™ 282

Catalog No. S11282

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  • Catalase solution
  • 1.2% Hydrogen peroxide (stabilized)
  • Phosphate buffer, pH 7.2 (20x concentrate)
  • Assay reagent (potassium iodide concentrate)
  • Acidification solution, 0.1M HCl
  • Color enhancer (concentrate)
  • Color developer (concentrate)
  • Plastic tube, 15mL
  • Comprehensive instructions, background material, and study questions



Methodology uses a colorimetric assay to measure the concentration of hydrogen peroxide (and therefore catalase activity) based on the oxidation of iodide by peroxide. This method differs from the AP Biology lab manual; it uses a safer system that eliminates the need for sulfuric acid.

  • Perform an enzyme assay
  • Determine the rate of a biochemical reaction



AP Biology Lab Kit 2: Principles of Enzyme Catalysis
Catalase solution, 1.2% Hydrogen Peroxide, 20X pH 7.2 Phosphate Buffer, Potassium Iodide concentrate assay reagent, 0.1M HCl acidification solution, color enhancer, color developer, 15mL Plastic tube, comprehensive instructions, background material and study questions
Understand enzyme catalysis and protein structure function relationships
30 to 45 min.
40 Students


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