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Edvotek™ Advanced Placement Laboratory Kit 2: Principles of Enzyme Catalysis
Understand enzyme catalysis and protein structure-function relationships.
Supplier: Edvotek™ 282
Includes:
- Catalase solution
- 1.2% Hydrogen peroxide (stabilized)
- Phosphate buffer, pH 7.2 (20x concentrate)
- Assay reagent (potassium iodide concentrate)
- Acidification solution, 0.1M HCl
- Color enhancer (concentrate)
- Color developer (concentrate)
- Plastic tube, 15mL
- Comprehensive instructions, background material, and study questions
Description
Methodology uses a colorimetric assay to measure the concentration of hydrogen peroxide (and therefore catalase activity) based on the oxidation of iodide by peroxide. This method differs from the AP Biology lab manual; it uses a safer system that eliminates the need for sulfuric acid.
- Perform an enzyme assay
- Determine the rate of a biochemical reaction
Specifications
| AP Biology Lab Kit 2: Principles of Enzyme Catalysis | |
| Catalase solution, 1.2% Hydrogen Peroxide, 20X pH 7.2 Phosphate Buffer, Potassium Iodide concentrate assay reagent, 0.1M HCl acidification solution, color enhancer, color developer, 15mL Plastic tube, comprehensive instructions, background material and study questions | |
| Refrigerated |
| Understand enzyme catalysis and protein structure function relationships | |
| 30 to 45 min. | |
| 40 Students |
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