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MilliporeSigma™ Chemicon™ Human iPS Selection Kit
Allows live cell imaging and identification of fully reprogrammed human induced pluripotent Stem cells from heterogeneous population of reprogramming intermediates. MilliporeSigma™; Chemicon™ Human iPS Selection Kit is a quick, easy and non-invasive method to monitor the pluripotent state of live human ESC and fully reprogrammed human iPS cells using immunocytochemistry (ICC).
Supplier: MilliporeSigma™ SCR502
Description
- Enables live selection of human iPS cells that can be further passaged and expanded for downstream applications
- Selected live stained human iPS clones maintain normal human ESC morphology and can proliferate and be passaged normally
- Fully reprogrammed human iPS cells possess following characteristics:
- They downregulate expression of fibroblast marker, CD13
- They upregulate expression of pluripotent markers, SSEA-4 and TRA-1-60
- They silence viral transgenes
- Activates endogenous expression of Nanog
- Assume HoeschstDim phenotype
- Kit Components:
- Anti-SSEA-4 PE (Mouse monoclonal IgG3), Cat. No. CS204438. One vial containing 50μg of 0.25mg/mL purified mouse IgG antibody
- Anti-TRA-1-60 FITC (Mouse monoclonal IgM), Cat. No. CS204460. One vial containing 50μg of 0.5mg/mL purified mouse IgM antibody
- Anti-CD13 PE (Mouse monoclonal IgG1), Cat. No. CS204449. One vial containing 50μg of 1mg/mL purified mouse IgG antibody
- Hoechst Nuclear Stain, Cat. No. CS204269. One vial containing 1μL of 1mg/mL stock solution
Specifications
Stable for 6 months at 2°to 8°C from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Maintain refrigerated at 2° to 8°C, protected from light in undiluted aliquots. | |
Immunocytochemistry | |
RUO |
Human iPS Selection Kit | |
1 Kit (Sufficient for 40 tests per antibody) |
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