MilliporeSigma™ Chemicon™ Endothelial Cell Characterization Kit

Enables researchers to characterize cultured endothelial cells in both constitutive and activated state using basic Immunocytochemistry techniques.

Supplier:  MilliporeSigma™ SCR023

Catalog No. SCR023

Description

Following antibody dilutions are recommended for immunocytochemistry:

• Mouse anti-Endothelial Cell (CD146): 1/500 dilution based on 1 mg/mL, final 2ng/mL
• Rabbit anti-VWF: 1/5000 dilution based on 1 mg/mL, final 0.2ng/mL
• Mouse anti-CD31: 1/50 dilution based on 0.1 mg/mL, final 20ng/mL

Following antibodies have been shown to stain cultured HUVEC after 4 to 6hr of TNF-alpha (100ng/mL) induction at following dilutions:

• Mouse anti-ICAM-1: 1/500 dilution based on 1 mg/mL, final 2ng/mL
• Mouse anti-VCAM-1: 1/500 dilution based on 1 mg/mL, final 2ng/mL

Specifications

• Content And Storage:
  • 50μg of Endothelial Cell (CD146) monoclonal (Part No. 2003789)
  • 50μg of CD31 monoclonal (Part No. 2003788)
  • 10μg of von Willibrand Factor (vWF) polyclonal (Part No. 2003787)
  • 50μg of VCAM-1 monoclonal (Part No.MAB2511-50UG)
  • 50μg of ICAM-1 monoclonal (Part No. 2003785)
  • 100μg of Mouse control IgG (Part No. PP54-100UG)
  • 100μg of Rabbit control IgG (Part No. PP64-100UG)
  Antibodies to ICAM-1, VCAM-1, von Willibrand Factor (vWF), and Endothelial Cells (CD146) are to be stored at 2° to 8°C (SCR023 - Part 1). Antibodies to CD31, Mouse IgG, and Rabbit IgG are to be stored at -20°C (SCR023 - Part 2).
• Product Type: Stem Cell Characterization Kit
• Quantity: 1 Kit

• Cell Type: Mouse Embryonic Stem Cells, Human Embryonic Stem Cells, Mesenchymal Stem Cells, Neural Stem Cells, Hematopoietic Stem Cells, Epithelial Cells, Pancreatic Stem Cells, Cardiac Stem Cells, Induced Pluripotent Stem Cells
• For Use With (Application): Immunocytochemistry

For Research Use Only