BD Retrievagen Antigen Retrieval Systems
Recommended for zinc-fixed and formalin-fixed paraffin embedded tissue sections that show suboptimal or no reactivity with antibodies for immunohistochemical staining
Supplier: BD 550524
10% neutral buffered formalin fixes the tissue by cross-linking proteins that may cause a conformational change or “masking” of the antigenic epitope leading to reduced or absent antibody reactivity. The BD Retrievagen Antigen Retrieval Systems are designed to unmask the epitope and cause the enhancement of antibody staining.
- Retrievagen A (pH 6.0) is provided as two separate solutions that need to be mixed
- Retrievagen B (pH 9.5) is provided as a ready-to-use solution
|25mL Retrievagen A Solution 1 and 100mL Retrievagen A Solution 2|
|Take 18 mL of BD Retrievagen A Solution 1 and 82 mL of BD Retrievagen Solution 2 and bring up the volume to 1 L with distilled Water|
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